c8.11.12 – Foliar terpenoids of Rosmarinus officinalis L. and its relationships to the attack of Alternaria alternata (Fr.) Keissl

Martini V (1), Capretti P (2), Comparini C (2), Scala A (2), Michelozzi M (1)

(1) IGV/CNR, v. Madonna del Piano 10, I-50019 Sesto Fiorentino (Firenze); (2) Dipartimento Biotecnologie Agrarie, Università di Firenze, p.le delle Cascine 28, I-50144 Firenze
Collocazione: c8.11.12 – Tipo Comunicazione: Poster
8° Congresso SISEF *
Sessione 11: “Poster” *

Contatto: Marco Michelozzi (marco.michelozzi@igv.cnr.it)

Abstract: Foliar terpenoids of Rosmarinus officinalis L. and its relationships to the attack of Alternaria alternata (Fr.) Keissl Valentina Martini1, Paolo Capretti2, Cecilia Comparini2, Aniello Scala2 and Marco Michelozzi1 1 Istituto di Genetica Vegetale – Consiglio Nazionale delle Ricerche, Via Madonna del Piano, I-50019 Sesto Fiorentino, Firenze, Italy. 2 Dipartimento Biotecnologie Agrarie, Sez. Protezione delle Piante, Università degli Studi di Firenze, Piazzale delle Cascine 28, I-50144, Firenze, Italy. Abstract: Conifers are prolific producers of oleoresin, which is a mixture of different classes of terpenoids and phenolics. Terpenoids are the largest family of plant compounds which exhibit enormous structural and functional diversity. About half of the resin produced by conifer species is composed by monoterpenes, and the other half by diterpenes, while sesquiterpenes occur in small amounts. The majority of terpenoids, such as monoterpenoids, sesquiterpenoids and diterpenoids are classified as “secondary” compounds. Numerous secondary terpenoids act as primary defensive chemicals against herbivorous and microbial attacks; however the production of these compounds has several other ecological functions which are indispensable for the survival of the species. Rosemary (Rosmarinus officinalis L.) is a plant widely distributed in Europe, Asia and Africa, and one of its elective growing area is the Mediterranean basin where spontaneous plants are diffusely distributed. The plant is widely known for its numerous applications in food field, but also for the increasing interest toward its pharmaceutical properties. The objective of these studies was to investigate the relationships between foliar monoterpene composition and the susceptibility to Alternaria alternata (Fr.) Keissl. in R. officinalis. Results showed that 1,8-cineole (37,93%), ( )-alpha-pinene (28,46%), and (-)-alpha-pinene (6%) were major components in the foliar tissue samples of rosemary. Most of the compounds did not show significant variations in the relative content between samples collected from the same clones planted on three sites that differ in environmental conditions. Changes in monoterpene composition were observed between juvenile and mature foliar tissues, while the terpene percentages in these mature tissues remained stable through seasons. In-vitro the major antifungal activities were observed for ( )-alpha -pinene, (-)-alpha -pinene, ( )-limonene, (-)-limonene, (-)-beta-pinene, and myrcene. Discussion is focused on the potential roles of variations in these constitutive terpenes and the attack of this pathogenic fungus and the possibility to use terpene markers as an aid in selecting for rosemary plants which could be less sensitive to the attack of A. alternata.

Parole chiave: Rosmarinus officinalis, Terpenoids, Alternaria alternata, Essential Oil

Citazione: Martini V , Capretti P , Comparini C , Scala A , Michelozzi M (2011). Foliar terpenoids of Rosmarinus officinalis L. and its relationships to the attack of Alternaria alternata (Fr.) Keissl . 8° Congresso Nazionale SISEF, Rende (CS), 04 – 07 Ott 2011, Contributo no. #c8.11.12