c8.10.10 – Differences in cadmium tolerance: general consideration on metallothioneins induction in Lux and Poli poplar clones

Cocozza C*, Caprari C, Palumbo G, Colombo C, Tognetti R

Università del Molise
Collocazione: c8.10.10 – Tipo Comunicazione: Poster
8° Congresso SISEF *
Sessione 10: “Poster” *

Contatto: Claudia Cocozza (claudia.cocozza@unimol.it)

Abstract: Poplar trees are known to be good candidates for bio-remediation, mainly for their high growth rates, an extensive root system and large biomass production. Plants respond to heavy metal toxicity in a variety of different ways. Tolerance mechanisms include exclusion, chelation and compartmentalization, by intracellular sequestration, of the metal ions. Metallothioneins (MTs) are low molecular mass cysteine-rich proteins that can bind heavy metals and may play a role in their intracellular sequestration. In an effort to understand processes that are related to heavy metal sequestration, the present work aims to investigate the mechanisms involved in plant response to cadmium excess and to evaluate the potentiality of poplars in the phytoremediation of polluted soils. To accomplish these goals cuttings of two poplar clones, Poli (Populus nigra L.) and Lux (Populus deltoides Bartram ex Marsh.) were studied for understanding the molecular and morphological basis underlying the mechanisms of heavy metal detoxification. Here we report the analysis of the expression profile by RT-PCR of six metallothionein genes carried out on cDNA synthesized from leaf and root of Poli and Lux woody cuttings treated with two different cadmium concentrations (50, 150 mg Cd kg-1 dry soil). The ability of plants to remove the pollutant from soil was also investigated considering the complex of soil and plant system.

Parole chiave: Phytoremediation, Populs, Cadmium, Metallothionein Genes

Citazione: Cocozza C, Caprari C, Palumbo G, Colombo C, Tognetti R (2011). Differences in cadmium tolerance: general consideration on metallothioneins induction in Lux and Poli poplar clones . 8° Congresso Nazionale SISEF, Rende (CS), 04 – 07 Ott 2011, Contributo no. #c8.10.10