c7.2.10 – Metal distribution and localization in poplar and willow clones treated with cadmium in hydroponics

Cocozza C* (1), Maiuro L (2), Martino D (1), Tognetti R (1)

(1) Laboratorio ECOGEOFOR, Dipartimento STAT, Università degli Studi del Molise; (2) Dipartimento STAM, Università del Molise
Collocazione: c7.2.10 – Tipo Comunicazione: Presentazione orale
7° Congresso SISEF *
Sessione 2: “Agroselvicoltura ed arboricoltura” *

Contatto: Claudia Cocozza (claudia.cocozza@unimol.it)

Abstract: To assess the potential for cadmium (Cd) uptake of some poplar and willow clones, a hydroponic screening for metal tolerance, accumulation and translocation was performed. Rooted cuttings were exposed for 3 weeks to 50 μM Cd sulphate in a growth chamber and morpho-physiological parameters and Cd content distribution in various parts of the plant were evaluated. The aim of this study was to discriminate 4 poplar and 2 willow clones in their response to high Cd concentrations in the growth solution and to show the potential of poplar and willow in the phytoremediation of Cd-contaminated substrate. Scanning electron microscopy equipped with energy-dispersive x-ray microanalysis provided information on element localization and chemical composition of biological samples. Scanning electron microscopy allows for the observation of samples, at high magnifications and good field depth with a minimum preparation, with the possibility to combine structural and analytical information by energy-dispersive x-ray microanalysis and digital images. Energy-dispersive x-ray microanalysis it was useful in establishing distribution maps of potentially toxic elements inside cells and tissues, despite its relatively low detection limits. Tissue concentration and localization of Cd (and other elements) in root tips of these clones were investigated, using ambient temperature scanning electron microscope both coupled with energy-dispersive x-ray microanalysis. These techniques were useful to investigate structural modifications and to identify in situ concentration and distribution of Cd in poplar and willow roots, establishing indirect correlations between accumulation and localization of the metal. Overall, observations suggested differential patterns between species and clones in accumulating Cd within the root profile. The possibility that these accumulation patterns arise from differences in uptake processes and structural properties is discussed and related to tolerance mechanisms.

Citazione: Cocozza C , Maiuro L , Martino D , Tognetti R (2009). Metal distribution and localization in poplar and willow clones treated with cadmium in hydroponics . 7° Congresso Nazionale SISEF, Isernia – Pesche (IS), 29 Set – 03 Ott 2009, Contributo no. #c7.2.10